[ORAL MEDICIME] 열처리한 치아에서 미토콘드리아 DNA와 핵 DNA 분석
[ORAL MEDICIME] Analysis fo Mitochondiral Nuclear DNA from Heated Teeth
소속 상세정보
안종모
허웅/윤창륙
KMID : 0355220040290020135
Abstract
The teeth are the hardest substances in the human body and stable tissue to a variety of environmental change such as heat and putrefaction. Therefore. they are forensically significant material for individual identification.
The auther conducted the experiment to analysis mitochondrial DNA(mtDNA) and nuclear DNA on heated teeth.
The experiment was done on 16 teeth of unrelated Koreans. they are classified 4 groups (100°C, 200°C, 300°C, 400°C by temperature) each 4 teeth and heated for lhour. DNA was extracted from each tooth and amplified by polymerase chain reaction.
The following is the result:
We can observed amplified product of hypervariable control region II of mtDNA on all teeth. And amelogenin gene and seven STR loci[CTT(CSFIPO. TPOX. THOU. FFV(F13A01, FESFPS. vWA), F13B] are amplified only 4 teeth heated on temperature of 100°C. .
As a result. we know that the mtDNA is extracted high temperature than nuclear DNA. And mtDNA analysis for individual identification is useful method especially when nuclear DNA is not extracted in charred body in high temperature.
키워드
원문 및 링크아웃 정보
등재저널 정보